This work describes a method to produce microstructured PDMS stamps that are metallized in two isolated sections, thus creating the possibility to electroprint a surface using such a stamp as both counter electrode and reference electrode in a surface contact electrochemical cell controlled using a potentiostat. This electromicrocontact printing method was used to perform patterned electroactivation of thiolated surfaces. Patterned electrooxidation of thiols to reactive thiolsulfinates/thiolsulfonates allowed for specific immobilization of thiolated polystyrene particles, showing the potential of this technique to be employed in selective covalent attachment of biomolecules through disulfide bonds.