In recent years, utilization of yeast and live bacterial culture for animal feed has increased. Supplementation of yeast culture beneficially affects the livestock by improvement of intestinal microbial balance, promotion of immune reaction and diminution of cholesterol level, etc. Yeast cells have contributed to the nutritional value of fermented foods, like bread and beers. Yeasts are good sources of protein or amino acids. Approximately 40 % of the weight of dried yeast consists of protein. In this study, the optimal cultivation conditions of the submerged culture for the cell production of S. cerevisiae were investigated. In the submerged culture, the optimal conditions were pH 4.5, 36 ℃, 6.0 % high fructose syrup and 2.0 % corn steep liquor. Under these optimal conditions, S. cerevisiae over 3.5 × 10⁸ cell per mL was obtained as a final product.