Chitosan is the second most plentiful biomass and is already known as a biocompatible, biodegradable, and low-toxic material[1]. In this work, low molecular and water-soluble chitosan (LMWSC, molecular weight of 22kDa) was characterized and evaluated as a gene carrier. The formation of plasmid/LMWSC complex was proved and transfection efficiency in 293T cells was evaluated. The cytotoxicity of LMWSC also was determined by MTT assay. To facilitate the cellular uptake by artery wall cells, artery wall binding peptide(AWBP) was introduced into LMWSC based gene carrier. AWBP-PEG-g-LMWSC was synthesized by conjugation the AWBP to the end of PEG-g-LMWSC. The synthesized AWBP-PEG-g-LMWSC can be considered as a promising gene carrier to treat cardiovascular related disease. Characteristics of the synthesized AWBP-PEG-g-LMWSC were analyzed by measuring FT-IR, ¹H-NMR, zeta-potentiometer, and AFM.