화학공학소재연구정보센터
홈 로그인 로그아웃 연락처 사이트맵
Journal of the Korean Industrial and Engineering Chemistry, Vol.15, No.8, 952-955, December, 2004
Export Citation
초음파법으로 제조된 은 나노 입자의 항균특성 연구
Study on the Antibacterial Effects of Silver Nanoparticles Prepared by Sonication Method
조경환†, 박수길
  • 충북대학교 공업화학과, 충북 361-763
Kyung Hwan Cho†, and Soo-Gil Park
  • Department of Industrial Chemical Engineering, Chungbuk National University, 12, Kaeshin, Heungduk, Cheongju, Chungbuk 361-763, Korea
E-mail:
초록
본 연구에서는 염화은(AgClO4)을 초음파 처리하여 얻은 10 nm 이하의 은 나노입자의 항균력을 측정하기 위해 그램 양성균인 Staphylococcus aureus (S. aureus)와 그램 음성균인 Escherichia coli (E. coli)를 배양한 배지에 0.054%은 나노용액 100 μL를 넣고 cup diffusion method로 실험을 행하였다. 그 결과 poly(N-vinyl 2-pyrrolidone) [PVP]로 안정화시킨 은 나노 입자에서만 항균활성을 보였으며 S. aureus와 E. coli에 대한 최소 억제농도(minimum inhibitory concentration, MIC)는 5 ppm이었으며 완전 살균농도는 50 ppm과 100 ppm이었다. 은 나노입자에 의해 사멸된 균의 형태상 변화와 세포 손상을 scanning electron microscopy (SEM)으로 확인하였다. 또한 은 나노 입자가 양성균(황색포도상 구균)과 음성균(대장균)에서의 특수한 향균 효과를 증명할 수 있었다.
The antibiological effect of nanoparticles on staphylococcus aureus (S. aureus) and Escherichia coli (E. coli) using silver nanoparticles (Ag-NPs), < 10 nm, prepared by the sonication of AgClO4 was studied. To determine the antibacterial activity, we applied 100 μL of 0.054% Ag-NPs solution to each culture medium containing test organism and evaluated the antibacterial activities by the cup diffusion method. Only Ag-NPs stabilized with poly(N-vinyl 2-pyrrolidone) [PVP] showed antibacterial activity and the minimum inhibitory concentration (MIC) to S. auresu and E. coli were 5 ppm. And the complete sterilizing concentrations for the test organisms were 50 and 100 ppm, respectively. Also we observed the morphological alteration and cell damages caused by Ag-NPs treatment using a scanning electron microscopy (SEM). Above results supports the evidence that Ag-NPs have strong antibacterial activity to S. aureus and E. coli.
Keywords:sonication;S. aureus;E. coli;silver nano-particle;anti bacterial effect
  1. Kim BK, Choi CJ, Machine and Materials, 14, 16 (2002)
  2. Won JO, Kang YS, J. Ind. Eng. Chem., 3, 24 (2000)
  3. Nagata Y, Watanabe Y, Fujita S, Taguchi S, J. Chem. Soc. Chem. Commun., 1620 (1992) 
  4. Yeung SA, Hobson R, Biggs S, Grieser F, J. Chem. Soc. Chem. Commun., 378 (1993) 
  5. Fujimoto T, Terauchi S, Umehara H, Kojima I, Henderson W, Chem. Mater., 13, 1057 (2001) 
  6. Kim TN, Feng QL, Kim JO, Wu J, Wang H, Chen GC, Cui FZ, J. Mater. Sci. -Mater. Med., 9, 129 (1998) 
  7. Uchida M, Chem. Ind., 46, 48 (1995)
  8. Feng QL, Wu J, Chen GQ, Cui FZ, Kim TN, Kim JO, Biomed. Mat. Res., 52, 662 (2000) 
  9. Grier N, Silver and Its Compounds, Disinfection, Sterilization and Preservation, 375, Lea and Febiger, Philadelphia (1983)
  10. Liau SY, Read DC, Pugh WJ, Furr JR, Russell AD, Lett. Appl. Microbiol., 25, 279 (1997) 
  11. Lehninger AL, Nelson DL, Cox MM, Principles of Biochemistry, 2nd ed. New York: Worth (1973)
  12. Davies RI, Etris SF, Catal. Today, 35, 87 (1997)
  13. Wells TN, Scully P, Paravincini G, Biochemistry, 34, 7896 (1995) 
  14. Colloids and Clusters, ed. G. Schmid, VHC Press, New York (1995)