Anion exchange groups(DEA) were introduced into porous hollow fiber membranes by radiation-induced ploymerization. A DEA group-containing polymer chain grafted onto a porous PE membrane. The BSA solution was forced to permeate across the hollow fiber DEA membrane. BSA was immobilized by the permeation of a BSA solution through the pore of the DEA membrane. The immobilized BSA was cross-linking with glutaraldehyde to prevent leakage. Afterwards, The GA 0.05 percent membrane having higher adsorption capacity than 0.025, 0.075, 0.1 percent GA concentration.