Release of Calcein from Temperature-Sensitive Liposomes in a Poly(N-isopropylacrylamide) Hydrogel

Hee Dong Han; Tae Woo Kim; Byung Cheol Shin; Ho Suk Choi

Macromolecular Research, Vol.13, No.1, 54-61, February, 2005

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Release of Calcein from Temperature-Sensitive Liposomes in a Poly(N-isopropylacrylamide) Hydrogel

Hee Dong Han, Tae Woo Kim , Byung Cheol Shin , and Ho Suk Choi^{1, †}

Advanced Materials Division, Korea Research Institute of Chemical Technology, P.O. Box 107, Yuseong, Daejeon 305-606, Korea
¹Department of Chemical Engineering, Chungnam National University, 220 Gung, Yuseong, Daejeon 305-764, Korea

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We prepared temperature-sensitive liposomes (TS-liposomes) modified with a thermosensitive polymer, such as poly(N-isopropylacrylamide) (PNIPAAm), to increase the degree of drug release from liposomes at the hyperthermic temperature. A PNIPAAm hydrogel containing TS-liposomes was also prepared to obtain a hydrogel complex at body temperature. In addition, a depot system for local drug delivery using the polymer hydrogel was developed to enhance therapeutic efficacy and prevent severe side effects in the whole body. The PNIPAAm-modified TS-liposome was fixed into the PNIPAAm hydrogel having a high temperature-sensitivity. The release behavior of calcein, a model drug, from TS-liposomes in the PNIPAAm hydrogel was then initiated by external hyperthermia; the results indicated that sustained release as a function of temperature and time was caused by the thermosensitivity of the liposome surface and diffusion of the drug into the PNIPAAm hydrogel. Our results indicated that TS-liposomes in a PNIPAAm hydrogel represented a plausible system for local drug delivery.

Keywords:poly(N-isopropylacrylamide);hydrogel;liposome;calcein;depot system

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[Related Articles]

[1] Kono K, Adv. Drug Deliv. Rev., 53, 307 (2001)

[2] Needham D, Dewhirst MW, Adv. Drug Deliv. Rev., 53, 285 (2001)

[3] Kong G, Anyarambhatla G, Petros WP, Braun RD, Colvin OM, Needham D, Dewhirst MW, Cancer Res., 60, 6950 (2000)

[4] Ishida O, Maruyama K, Yanagie H, Eriguchi M, Iwastru M, Jpn. J. Cancer Res., 91, 118 (2000)

[5] Mayer DE, Shin BC, Kong GA, Dewhirst MW, Chilkoti A, J. Control. Release, 74, 213 (2001)

[6] Lee JW, Lee DS, Kim SW, Macromol. Res., 11(3), 189 (2003)

[7] Lee SB, Seo SM, Lim YM, Cho SK, Lee YM, Macromol. Res., 12(3), 269 (2004)

[8] Kim KH, Shin YJ, Polym.(Korea), 18(3), 412 (1994)

[9] Park YS, Han HD, Hong SU, Kim SS, Shin BC, Polym.(Korea), 28(1), 59 (2004)

[10] Shin BC, Kim SS, Ko JK, Jegal J, Lee BM, Eur. Polym. J., 39, 579 (2003)

[11] Tung LH, Fractionation of Synthetic Polymers, Marcel Dekker, New York, p. 103. (1977)

[12] Kono K, NAakai R, Morimoto K, Takagishi T, Biochim. Biophys. Acta, 1416, 239 (1999)

[13] Needham D, Anyarambhatla G, Kong G, Dewhirst MW, Cancer Res., 60, 1197 (2000)

[14] Hayashi H, Kono K, Takagishi T, Bioconjugate Chem., 10, 412 (1999)

[15] Mayer LD, Hope MJ, Cullis PR, Janoff AS, Biochim. Biphys. Acta, 817, 193 (1985)

[16] Hope MJ, Bally MB, Webb G, Cullis PR, Biochim. Biophys. Acta, 812, 55 (1985)

[17] Lopez O, delaMaza A, Coderch L, Lopez-Iglesias C, Wehrli E, Parra JL, Febs Lett., 426, 314 (1998)

[18] Hayashi H, Kono K, Takagishi T, Biochem. Biophys. Acta, 1280, 127 (1996)

[19] Leroux JC, Garrec E, Hong DL, Drummond DC, J. Control. Release, 72, 71 (2001)

[20] Ringsdorf H, Venzmer J, Winnik FM, Chem. Int. Ed. Engl., 30, 315 (1991)

[21] Ringsdorf H, Sackmann E, Simon J, Winnik FM, Biochim. Biophys. Acta, 1153, 335 (1993)

[22] Hermanson GT, Bioconjugate Techniques, Academic Press, San Diego, pp 305-307 (1996)

[23] Polozova A, Yamazaki A, Brash JL, Winnik FM, Colloids Surf. A: Physicochem. Eng. Asp., 147, 17 (1999)

[24] Yoshida R, Uchida K, Kaneko Y, Sakai K, Kikuchi A, Sakurai Y, Okano T, Nature, 374(6519), 240 (1995)

[25] Suisha F, Kawasaki N, Miyazaki S, Shirakawa M, Yamatoya K, Sasaki M, Attwood D, Int. J. Pharm., 172, 27 (1998)

[26] Paavola A, Kilpelainen I, Yliruusi J, Rosenberg P, Int. J. Pharm., 199, 85 (2000)