Biotechnology Letters, Vol.26, No.4, 311-315, 2004
Construction of a high-biomass, iron-enriched yeast strain and study on distribution of iron in the cells of Saccharomyces cerevisiae
A high-biomass, iron-enriched Saccharomyces cerevisiae ZYF-15 was constructed by interspecies protoplast fusion. Under optimal fermentation condition, the biomass and iron content of the strain reached 11 g l(-1) and 25 mg Fe g(-1) dry cells, respectively. About 96% of enriched iron is converted into organic iron, which is mainly in cell walls and vacuoles with some bound to DNA, RNA and protein.