The bioremediation of 2,4,6-trinitrotoluene (TNT) contaminated soil was performed on a laboratory scale. To compare bioremediation methods, a soil slurry reactor and a soil column reactor were operated and the effects of supplemental sources were investigated. Optimal conditions for the two bioremediation systems for the removal of TNT were obtained. In the soil slurry reactor, about 60% of the 1000 mg/kg TNT contaminated soil was degraded after 10 d, nearly complete biodegradation (> 99%) was achieved within 25 d, and the microorganisms grew and reached a maximum of 9.5 x 10(9) CFU/ml at 15 d. In the soil column reactor, about 50% of the 1000 mg/kg TNT contaminated soil was degraded after 25 d and nearly complete biodegradation (>99%) was achieved within 60 d. Microorganisms grew and reached a maximum of 9.8 x 10(10) CFU/g soil at 40 d. These results should help in determining the best bioremediation method and improving the design and operation of large scale clean up of contaminated sites by bioremediation systems.