Journal of Bioscience and Bioengineering, Vol.97, No.2, 134-137, 2004
Accumulation of S-2-hydroxyethyl derivatives of L-cysteine and L-homocysteine by a methionine auxotroph of Escherichia coli with the addition of 2-mercaptoethanol to the culture
We previously reported [J. Biosci. Bioeng., 94, 178-181 (2002)] that an Escherichia coli MetC-deficient mutant can accumulate L-cystathionine. When 2-mercaptoethanol was added to the culture medium during fermentation, the accumulation of L-cystathionine decreased and S-(2-hydroxyethyl)-L-cysteine and S-(2-hydroxyethyl)-L-homocysteine were accumulated.
Keywords:L-cystathionine;cystathionine beta-lyase;2-mercaptoethanol;S-(2-hydroxyethyl)-cysteine;S-(2-hydroxyethyl)-homocysteine]