Inter-simple sequence repeat markers and flow cytometry for the characterization of closely related Citrus limon germplasms

Capparelli R; Viscardi M; Amoroso MG; Blaiotta G; Bianco M

Biotechnology Letters, Vol.26, No.16, 1295-1299, 2004

DOI10.1023/B:BILE.0000044920.36497.fc Export Citation

Inter-simple sequence repeat markers and flow cytometry for the characterization of closely related Citrus limon germplasms

Capparelli R, Viscardi M, Amoroso MG, Blaiotta G, Bianco M

The genetic relationship among commercial cultivars of Citrus limon (lemon) was analysed by inter-simple sequence repeats (ISSR) and flow cytometry techniques. Two cultivars with a close germplasm were distinguished by screening 10 SSR primers and by measuring DNA content of prestained nuclei.

Keywords:Citrus limon;flow cytometry;inter-simple sequence repeats;nuclear DNA content;specific sequence characterized amplified region (SCAR)