We recently proposed a technique for preparing monodisperse emulsions with a coefficient of variation below 5% from a silicon array of micrometer-sized channels perpendicular to the plate surface, named a straight-through microchannel (MC). This study involved three-dimensional computational fluid dynamics (CFD) simulations to calculate the formation of an oil-in-water (O/W) emulsion droplet from straight-through MCs with circular and elliptic cross sections. The CFD results demonstrated that the oil phase that passed through the elliptic MCs exceeding a threshold aspect ratio between 3 and 3.5 was cut off spontaneously into a small droplet with a diameter of similar to40 mum. Sufficient space for water at the channel exit had to be maintained for successful droplet formation. The formation and shrinkage of a neck inside the channel caused an increased pressure difference inside the channel and an increased velocity value near the neck. The pressure and velocity values at the neck drastically changed, and the neck was cut off instantaneously just before the completion of droplet formation. This process was triggered by a gradually increased pressure difference between the circular neck and inflating oil phase. The findings obtained in this paper provide useful numerical and visual information about the droplet formation phenomena from the straight-through MCs. The CFD results were verified by the experimental results, showing that the CFD approach can help design a suitable channel structure.