The optimization of process parameters for the production of an antifungal molecule produced by Bacillus licheniformis BC98 was carried out using novel statistical tools. The parameters studied were pH, temperature and agitation rate. Fed batch cultivations were carried out since the maximum production of the molecule was observed in the late log phase. The statistical design used allows the evaluation of the effects of several different process variables in a single batch. Data from several batches indicated that while the effects of two of the variable factors, viz., temperature and agitation rate, were significant at 95% confidence intervals, the agitation rate was most critical for the production of the molecule, and pH had no significant effect. The cultivation of the bacterium under optimized conditions (fed batch, 150 rpm, 32degreesC, pH 5.8) resulted in a 30-fold increase compared with that under unoptimized conditions (shake flask. 100 rpm, 29degreesC, pH 5.8) in the production of the antifungal molecule.