Biotechnology Letters, Vol.26, No.22, 1731-1737, 2004
Real Time RT-PCR and flow cytometry to investigate wheat kernel hardness: role of puroindoline genes and proteins
Developing seeds from Triticum aestivum (wheat) cultivars were collected after flowering and analysed for puroindoline a and b gene expression by Real Time RT-PCR. Mature seeds were investigated for the presence and the amount of starch-associated puroindoline a and b proteins by flow cytometry. Puroindoline a gene and protein were found to have a predominant role in controlling wheat kernel hardness.