Kinetic modeling is a powerful tool for combustion investigations and has been widely used. The validation of the model is a very important element of the work, to achieve realistic results, and it normally uses concentration profiles of some participant species. In this work. a kinetic model was validated using the chemiluminescent emissions of excited radicals produced in ethanol vapor combustion in a closed chamber, where a fixed mass of premixed reagents was ignited. A reaction mechanism made up of 128 reactions and 40 species was simulated with the SENKIN code of the CHEMKIN-III software package and it was able to reproduce with good accuracy the chemiluminescent profiles of OH*, HCO*, CH2O*, CH*, and C-2* species recorded experimentally from ethanol combustion. Analysis of the developed ethanol combustion mechanism showed that the CH3, radical is the main precursor to the chain reaction for the production of the most excited species.