We report the Mossbauer spectroscopy study on Fe-doped charge-ordering manganite Y0.5Ca0.5MnO3. The dynamic isomer shift is observed for charge-ordering manganite, and its origin may be due to strong JahnTeller distortions in Y0.5Ca0.5MnO3, causing electron-phonon coupling. The evolution of Mossbauer spectroscopy as a function of temperature shows two different phases with significantly different quadrupole splitting values below the charge-ordering transition temperature. This confirms that there exist two different Mn sites (i.e., Mn3+ and Mn4+ ions), which can be identified by the microscopic method of Mossbauer spectroscopy.