This article describes a surface micromachined cantilever beam-based resonator for biolo6cal sensing applications. The study used a novel microfabrication technique of merged epitaxial lateral overgrowth (MELO) and chemical mechanical polishing (CMP) to fabricate thin, low stress. single-crystal silicon cantilever beams. The vibration spectra of the cantilever beams. excited by thermal and ambient noise, was measured in air usina a Dimension 3100 Series scanning probe microscope (SPM), and in certain cases, a Polytec MSV300 laser Doppler vibrometer. The sensorss were used to detect the mass of Listeria innocua bacteria by applying increasing concentration of bacteria suspension on the same cantilever beams and measuring the resonant frequency changes in air. Cantilever beams were also used to detect the mass of proteins such as Bovine Serum Albumin (BSA) and antibodies for Listeria that were attached to the cantilever's surfaces by physical adsorption; following which they were used to capture and detect the mass of the bacterial cells on the functionalized cantilever beam surfaces'. The effects of critical point drying of the proteins were evaluated and the results indicate that the functionality of the antibodies was not reduced once rehydrated after critical point drying. The developed biosensor is capable of rapid and ultrasensitive detection of bacteria and promises significant potential for the enhancement of microbiological research and dia-nostics. (C) 2004 American Vacuum Society.