Crystallographic studies of Mycobacterium tuberculosis polyphosphate/ATP-NAD kinase complexed with NAD

Mori S; Yamasaki M; Maruyuma Y; Momma K; Kawai S; Hashimoto W; Mikami B; Murata K

Journal of Bioscience and Bioengineering, Vol.98, No.5, 391-393, 2004

Mori S, Yamasaki M, Maruyuma Y, Momma K, Kawai S, Hashimoto W, Mikami B, Murata K

NAD kinase from Mycobacterium tuberculosis (Ppnk) uses ATP or inorganic polyphosphate [poly(P)]. Ppnk overexpressed in Escherichia coli was purified and crystallized in the presence of NAD. Preliminary X-ray analysis of the resultant crystal indicate that the crystal belongs to hexagonal space group P6(2)22 and is holo-Ppnk complexed with NAD.

Keywords:NAD kinase;Mycobacterium tuberculosis;polyphosphate;NAD;ATP;crystallization