The SSU1-R gene provides high sulfite resistance to the wine yeast Y9-16B. In this study, we examined the distribution of this gene in 61 wine yeasts and 4 laboratory yeasts. We also analyzed the number of repeats of a 76-bp promoter sequence and its relationship to sulfite resistance. We found that the SSU1-R gene was present in 31 of the 61 wine yeasts. Furthermore, we found that the number of repeats in the promoter region of SSU1-R varied from two to six. Using RsaI, which cuts only once in the repeat, we suggested that the repeats all consisted of the 76-bp sequence. Finally, we found that there was a complex relationship between the number of repeats and sulfite resistance.