Polymer carriers with various degrees of hydrophobicity, porosity, and polarity were studied for immobilization of lipase from Candida rugosa. Copolymers and terpolymers were prepared by suspension polymerization of six monomers, ethyl and butyl acrylate, acrylonitrile, methyl methacrylate, 2-hydroxypropyl methacrylate and vinyl acetate, and three crosslinking agents, divinylbenzene, ethylene glycol dimethacrylate and trimethylolpropane triacrylate, in the presence of various diluents. Immobilization of lipase was carried out via glutaraldehyde chemical binding or by adsorption. It was found that the best results were obtained for carriers of butyl acrylate, ethyl acrylate or 2-hydroxypropyl methacrylate crosslinked with ethylene glycol dimethacrylate.