Chinese herbal medicine Curcuma longa is used in food, Chinese herbal medicine and parts of medicine. In particular, Curcuma longais also used as spice due to its unique fragrance, and curcumin substance, a Curcuma longa substance, is known as an outstanding anti-cancer substance. Thus, it is a Chinese herbal conducted an experiment, separating substances that are found in Curcuma longa. In particular, curcumin(CUR), activation material,and other effective substances were subjected to extraction, separation and purification to verify effective substances were subjected to extraction, separation and purification to verify effective substance index by production site by leveraging a method of analysis that uses HPLC. Moreover, this research separated herb medicine substances such as imported material and Curcuma aromatica to conduct comparative analysis of substances. Thus, this research was attempted to develop base data for the medicine development as well as identification of substances that may be used as additive for beverages, and to differentiate Korean made from imports. Towards this end, each chemical combination of separated substances are subjected to physical property, IR, 1H-NMR, GC Mass, HPLC spectrum analysis for publication.