Curcumin (CUR), activation material, is extracted, separated, and purified from Curcuma longa, other effective substances are extracted and separated to conduct anti-cancer test on effective substance among these substances. In particular, Curcumin is used as anti-oxidation, anti-inflammation, and anti-cancer materials. Thus, to check for existence (non-existence) of cancer cells and morphological change, SK-HEP-1, which is adenocarcinoma cell line, was used on 6 well, and 30,000 cells and Chang cell, which is normal cell line, were also used. Curcumin among substances is subjected to processing so that its final concentration levelstands at 10M, 1 mM, and change in cell line was observed. Movement of target subject to observation is as follows, which are subject to verification and publication. A: Chang cell's normal cell, B: change in cell form after 24 hours after processing 10M curcumin onto Chang cell, C: Sk-Hep-1 cell's normal cell,and D: growth andgrowth curtailing of cells subjected to 10M curcumin on SK-Hep-1 cell.