The matrix-free laser desorption/ionization of the biosamples for time-of-flight mass spectrometry from an array of gated silicon tips has been presented. The gated silicon tips were fabricated similar to the well-known electron-field-emitters gated sources. In this method, called desorption/ionization on silicon tips array, an array of gated silicon sharp tips covered with a biosample and irradiated by a pulsed ultraviolet laser serves as, the effective source of ions, replacing standard ionization platforms. Tests have confirmed that the effective laser desorption/ionization of the low-mass biosamples, such as dopamine and leucyl-glycyl-glycine tripeptide, may be obtained It is possible that the method will play a very important role in post-genetic proteomic investigations. (c) 2005 American Vacuum Society.