A noble RBC aggregometer with vibration-induced disaggregation mechanism

S. Shin; J.H. Jang; M.S. Park; Y.H. Ku; J.S. Suh

Korea-Australia Rheology Journal, Vol.17, No.1, 9-13, March, 2005

Export Citation

A noble RBC aggregometer with vibration-induced disaggregation mechanism

S. Shin^†, J.H. Jang, M.S. Park, Y.H. Ku, and J.S. Suh¹

School of Mechanical Engineering, Kyungpook National University, Korea
¹Dept. of Laboratory Medicine, School of Medicine, Kyungpook National University Hospital, 1370 Sangyeok-dong Buk-gu, Daegu 702-701, Korea

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The aggregation of red blood cells (RBCs) is a major determinant of blood flow resistance passing through various veins. Available techniques for measuring RBC aggregation often require pretreating and washing after each measurement, which is not optimal for day-to-day clinical use. A laser reflection technique has been combined with a vibration-aided disaggregation mechanism, which shows significant advances in aggregometer design, operation and data analysis. The essential features of this design are in its simplicity and a disposable element that is in contact with the blood sample. Using extremely small quantities of blood, the RBCs subjected to vibrations can be quickly and completely disaggregated. This is followed by measuring the backscattered light intensity. The measurements with the present sensor were compared with those of a commercial aggregometer and a strong correlation was found between them. The newly-developed optical aggregometer can measure the RBC aggregability difference between young and old cell suspension with ease and accuracy.

Keywords:aggregation;red blood cell;vibration;light;reflection

[References]

Baskurt OK, Temiz A, Meiselman HJ, J. Lab. Clin. Med., 130, 183 (1997)
Bauersachs RM, Shaw S, Zeidler A, Meiselman HJ, Clin. Hemorheol., 7, 432 (1987)
Boynard M, Lelievre JC, Guillet R, Biorheology, 24, 451 (1987)
Chien S, Jan KM, Microvasc. Res., 5, 155 (1973)
Dormandy L, Ernst E, Matrai A, Flute PT, Am. Heart. J., 104, 1364 (1982)
Hardeman MR, Goedhart PT, Dobbe JGG, Lettinga KP, Clin. Hemorheol., 14, 605 (1994)
Hein HJ, Bauersachs RM, Feinstein EI, MEiselman HJ, Clin. Hemorheol., 7, 430 (1987)
Meiselman HJ, baskurt OK, Sowemimo-Coker SO, Wenby RB, Biorheology, 36, 427 (1999)
Neu B, Meiselman HJ, Biophys. J., 83, 2482 (2002)
Pribush A, Meiselman HJ, Meyerstein D, Meyerstein N, Biorheology, 36, 411 (1999)
Rampling MW, Red cell aggregation and yield stress, in Clinical Blood Rheology Vol. 1, edited by G.D. Lowe, CRC Press, Boca Raton, Florida, USA. (1988)
Rampling MW, Meiselman HJ, Neu B, Baskurt OK, Biorheology, 41, 91 (2004)
Shvartsman LD, Fine I, SPIE proc., 4263, 122 (2001)
Shin S, Ku YH, Suh JS, Moon SY, Jang JY, Ann. Biomed. Eng., 31, 1077 (2003)
Shin S, Park MS, Jang JH, Ku YH, Suh JS, Korea-Aust. Rheol. J., 16(3), 129 (2004)
Shin S, Jang JH, Park MS, Ku YH, Suh JS, Rev. Sci. Instrum., 76 (2005)
Stoltz JF, Singh M, Riha P, Hemorheology in Practice. IOS Press, Amsterdam. (1999)
Zhao H, Wang X, Stolz JF, Clin. Hemorheol. Microcirc., 21, 297 (1999)

[Referenced By]

[1] Baskurt OK, Temiz A, Meiselman HJ, J. Lab. Clin. Med., 130, 183 (1997)

[2] Bauersachs RM, Shaw S, Zeidler A, Meiselman HJ, Clin. Hemorheol., 7, 432 (1987)

[3] Boynard M, Lelievre JC, Guillet R, Biorheology, 24, 451 (1987)

[4] Chien S, Jan KM, Microvasc. Res., 5, 155 (1973)

[5] Dormandy L, Ernst E, Matrai A, Flute PT, Am. Heart. J., 104, 1364 (1982)

[6] Hardeman MR, Goedhart PT, Dobbe JGG, Lettinga KP, Clin. Hemorheol., 14, 605 (1994)

[7] Hein HJ, Bauersachs RM, Feinstein EI, MEiselman HJ, Clin. Hemorheol., 7, 430 (1987)

[8] Meiselman HJ, baskurt OK, Sowemimo-Coker SO, Wenby RB, Biorheology, 36, 427 (1999)

[9] Neu B, Meiselman HJ, Biophys. J., 83, 2482 (2002)

[10] Pribush A, Meiselman HJ, Meyerstein D, Meyerstein N, Biorheology, 36, 411 (1999)

[11] Rampling MW, Red cell aggregation and yield stress, in Clinical Blood Rheology Vol. 1, edited by G.D. Lowe, CRC Press, Boca Raton, Florida, USA. (1988)

[12] Rampling MW, Meiselman HJ, Neu B, Baskurt OK, Biorheology, 41, 91 (2004)

[13] Shvartsman LD, Fine I, SPIE proc., 4263, 122 (2001)

[14] Shin S, Ku YH, Suh JS, Moon SY, Jang JY, Ann. Biomed. Eng., 31, 1077 (2003)

[15] Shin S, Park MS, Jang JH, Ku YH, Suh JS, Korea-Aust. Rheol. J., 16(3), 129 (2004)

[16] Shin S, Jang JH, Park MS, Ku YH, Suh JS, Rev. Sci. Instrum., 76 (2005)

[17] Stoltz JF, Singh M, Riha P, Hemorheology in Practice. IOS Press, Amsterdam. (1999)

[18] Zhao H, Wang X, Stolz JF, Clin. Hemorheol. Microcirc., 21, 297 (1999)