Preparation and Swelling Characteristics of Hydrogel from Microbial Poly(γ-glutamic acid) by γ-Irradiation

Seong-Hyun Choi; Kyung-Sook Whang; Jong-Soo Park; Woo-Young Choi; Min-Ho Yoon

Macromolecular Research, Vol.13, No.4, 339-343, August, 2005

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Preparation and Swelling Characteristics of Hydrogel from Microbial Poly(γ-glutamic acid) by γ-Irradiation

Seong-Hyun Choi, Kyung-Sook Whang¹, Jong-Soo Park², Woo-Young Choi³, and Min-Ho Yoon^{3, †}

The Institute of Natural Science, Korea
¹Division of Lifescience, Mokwon University, Deajeon 302-729, Korea
²Namyang Dairy Products Co., Ltd., Gongju 314-914, Korea
³Department of Agricultural Chemistry, College of Agriculture and Lifesciences, Chungnam National University, Daejeon 305-764, Korea

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Microbial hydrogel was prepared by γ-irradiation of poly(γ-glutamic acid) (PGA) which was produced from Bacillus subtilis BS 62 and it’s physico-chemical characteristic was examined. The hydrogel, prepared from 10% PGA with the dose of 48 kGy, was swollen up to 1,370 times of specific water content as dry weight basis. The hydrogels obtained above the dose of 48 kGy appeared to have higher compressive strength but lower specific water content. The period to reach a swelling equilibrium for the hydrogel in deionized water at the temperature range of 4 to 45 ℃ was about 10 h. The swollen hydrogel was shrunk in ionic solutions with the increase of ionic strength, and the rate of shrinkage was greater in calcium chloride solution than in sodium chloride. Specific water content of the hydrogel was quickly decreased at 80 ℃, showing a thermally hydrodegradable property.

Keywords:poly(γ-glutamic acid);PGA;Bacillus subtilis BS 62;γ-irradiation;hydrogel;biopolymer

[References]

Choi HJ, Kunioka M, Radiat. Phys. Chem., 46, 175 (1995)
Lenaert V, Gurny R, in Bioadhesive Drug Delivery Systems, Boca raton (FL), CRC Press, pp 1-24. (1990)
Scotchford CA, Cascone MG, Downes S, Giusti P, Biomaterials, 19, 1 (1998)
Kishi R, Ichijo H, Hirasa O, J. Intell. Mater. Syst. Struct., 4, 533 (1993)
Peppas NA, Merrill EW, J. Polym. Sci., 14, 441 (1976)
Hoffman AS, J. Control. Release, 6, 297 (1987)
Wardand JH, Peppas NA, J. Control. Release, 71, 183 (2001)
Kamathand KR Park K, Adv. Drug Deliv. Rev., 11, 59 (1993)
Kunioka M, Choi HJ, Polym. Degrad. Stabil., 59, 33 (1998)
Choi SH, Park JS, Whang KS, Yoon MH, Choi WY, Agric. Chem. Biotechnol., 47, 60 (2004)
Hara T, Shiraishi A, Fujii H, Ueda S, Agric. Biol. Chem., 48, 2373 (1984)
Hara T, Choi SH, Choi WY, J. Microbiol. Biotechnol., 11, 342 (2001)
Ricka J, Tanaka T, Macromolecules, 17, 2916 (1984)
Markland P, Amidon GL, Yang VC, Int. J. Pharm., 178, 183 (1999)
Choi HJ, in Characterization of Hydrogel Prepared by γ-irradiation from Microbial Poly(γ-glutamic acid), Ph.D. thesis in the graduate school, Yonsei University, Korea (1996)
Fan K, Gonzales D, Sevoian M, J. Environ. Polym. Degrad., 4, 253 (1996)

[Referenced By]

[1] Choi HJ, Kunioka M, Radiat. Phys. Chem., 46, 175 (1995)

[2] Lenaert V, Gurny R, in Bioadhesive Drug Delivery Systems, Boca raton (FL), CRC Press, pp 1-24. (1990)

[3] Scotchford CA, Cascone MG, Downes S, Giusti P, Biomaterials, 19, 1 (1998)

[4] Kishi R, Ichijo H, Hirasa O, J. Intell. Mater. Syst. Struct., 4, 533 (1993)

[5] Peppas NA, Merrill EW, J. Polym. Sci., 14, 441 (1976)

[6] Hoffman AS, J. Control. Release, 6, 297 (1987)

[7] Wardand JH, Peppas NA, J. Control. Release, 71, 183 (2001)

[8] Kamathand KR Park K, Adv. Drug Deliv. Rev., 11, 59 (1993)

[9] Kunioka M, Choi HJ, Polym. Degrad. Stabil., 59, 33 (1998)

[10] Choi SH, Park JS, Whang KS, Yoon MH, Choi WY, Agric. Chem. Biotechnol., 47, 60 (2004)

[11] Hara T, Shiraishi A, Fujii H, Ueda S, Agric. Biol. Chem., 48, 2373 (1984)

[12] Hara T, Choi SH, Choi WY, J. Microbiol. Biotechnol., 11, 342 (2001)

[13] Ricka J, Tanaka T, Macromolecules, 17, 2916 (1984)

[14] Markland P, Amidon GL, Yang VC, Int. J. Pharm., 178, 183 (1999)

[15] Choi HJ, in Characterization of Hydrogel Prepared by γ-irradiation from Microbial Poly(γ-glutamic acid), Ph.D. thesis in the graduate school, Yonsei University, Korea (1996)

[16] Fan K, Gonzales D, Sevoian M, J. Environ. Polym. Degrad., 4, 253 (1996)