아임계/초임계 유체를 이용한 콩에 포함된 Daidzein과 Genistein의 추출특성

최두영; 鄭金珠; 노경호; Du Young Choi; Jinzhu Zheng; Kyung Ho Row

Journal of the Korean Industrial and Engineering Chemistry, Vol.16, No.5, 609-613, October, 2005

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아임계/초임계 유체를 이용한 콩에 포함된 Daidzein과 Genistein의 추출특성

Characteristics of Extraction of Daidzein and Genistein in Soybean Using Sub/Supercritical Fluids

최두영, 鄭金珠, 노경호 ^†

인하대학교 화학공학과, 초정밀생물분리기술연구센터

Du Young Choi, Jinzhu Zheng, and Kyung Ho Row^†

Center for Advanced Bioseparation Technology and Department of Chemical Engineering, Inha University, Incheon 402-751, Korea

E-mail:

초록

토종콩에 포함된 daidzein과 genistein을 초임계 CO2와 아임계/초임계 H2O를 이용하여 추출하였다. 추출액은 역상 고성능 액체 코토마토그래피(RP-HPLC)를 사용하여 분석하였다. 초임계 유체의 온도와 압력, 에탄올 농도의 변화에 따라서 비배당체의 체류시간, 체류인자, 컬럼효율, 컬럼의 선택도, 분리도를 비교하였다. 초임계 CO2를 사용하였을 때, daidzein과 genistein의 추출특성은 온도와 에탄올농도의 영향을 주로 받았다. 아임계/초임계 H2O를 사용하였을 때는 초임계 상태의 400 ℃, 250 bar에서의 추출효율이 가장 우수하엿다. 일반적으로 초임계 CO2를 사용하는 경우, 초임계 H2O에 비해서 약 10배 이상 비배당체의 추출수율이 증가하였다.

Daidzein and genistein were extracted from Korean soybean by supercritical CO2 and sub/supercritical water. The extracted sample was analyzed by reversed-phase high performance liquid chromatography (RP-HPLC). The retention time, retention factor, column efficiency, column selectivity and resolution of aglycons were compared with the change in the temperature and pressure of supercritical fluid and ethanol concentration. The characteristics of extraction of daidzein and genistein were more affected by ethanol concentration using supercritical CO2. The most desirable extraction yield was obtained by supercritical H2O with 400 ℃ and 250 bar. Generally, the extraction yield of aglycons increased over 10 times using supercritical CO2 than sub/supercritical H2O.

Keywords:characteristics;extraction;daidzein;genistein;sub/supercritical fluid

[References]

Brunner G, J. Food Eng., 67, 21 (2005)
Mccabe WL, Smith JC, Harriott P, Unit operations of chemical engineering, 6th ed., McGraw-Hill, New York (2001)
Boo SJ, Byun SY, J. Biotechnol. Bioeng., 14, 490 (1999)
Han SK, Lee KJ, Kim JD, Lee WY, Row KH, Korean Chem. Eng. Res., in press, 47 (2004)
Macias-Sanchez MD, Mantell C, Rodriguez M, MartinezdelaOssa E, Lubian LM, Montero O, J. Food Eng., 66, 245 (2005)
Song MS, Lee YW, Kim JD, Row KH, HWAHAK KONGHAK, 41(2), 207 (2003)
Lee KJ, Choi DY, Row KH, HWAHAK KONGHAK, 41(5), 612 (2003)
Boo SJ, Byun SY, J. Biotechnol. Bioeng., 16, 95 (2001)
Bahram H, Arjmandi B, Smith J, J. Nutr. Biochem., 13, 130 (2002)
Vendula K, Jana P, Michael b, Theriogenology, 61, 1307 (2004)
Row KH, Principles and Applications of Liquid Chromatography, Inha University Press, Incheon (1999)

[Related Articles]

[1] Brunner G, J. Food Eng., 67, 21 (2005)

[2] Mccabe WL, Smith JC, Harriott P, Unit operations of chemical engineering, 6th ed., McGraw-Hill, New York (2001)

[3] Boo SJ, Byun SY, J. Biotechnol. Bioeng., 14, 490 (1999)

[4] Han SK, Lee KJ, Kim JD, Lee WY, Row KH, Korean Chem. Eng. Res., in press, 47 (2004)

[5] Macias-Sanchez MD, Mantell C, Rodriguez M, MartinezdelaOssa E, Lubian LM, Montero O, J. Food Eng., 66, 245 (2005)

[6] Song MS, Lee YW, Kim JD, Row KH, HWAHAK KONGHAK, 41(2), 207 (2003)

[7] Lee KJ, Choi DY, Row KH, HWAHAK KONGHAK, 41(5), 612 (2003)

[8] Boo SJ, Byun SY, J. Biotechnol. Bioeng., 16, 95 (2001)

[9] Bahram H, Arjmandi B, Smith J, J. Nutr. Biochem., 13, 130 (2002)

[10] Vendula K, Jana P, Michael b, Theriogenology, 61, 1307 (2004)

[11] Row KH, Principles and Applications of Liquid Chromatography, Inha University Press, Incheon (1999)