In this study we evaluated the performance of rock wool as a biofilter medium for the removal of hydrogen sulfide and compared it with other common organic packings, including compost and woodchip media. The biofilter packed with compost achieved > 99 % removal efficiencies at an empty bed residence time of 30 sec, but showed a higher pressure drop over the 80-day study period. In the wood chip biofilter, low removal efficiencies (ca. 62%) and minimal microbial accumulation were observed at a longer residence time (80 sec). On the other hand, the rock wool biofilter provided high removal efficiencies (>96 %), a maximum elimination capacity of 1115 g-Sulfur/m3 bed/day, and a relatively low pressure drop. Our findings indicate that this material is suitable and effective for the treatment of hydrogen sulfide.