Surface Characteristics and Fibroblast Adhesion Behavior of RGD-Immobilized Biodegradable PLLA Films

Hyun Jung Jung; Kwang-Duk Ahn; Dong Keun Han; Dong-June Ahn

Macromolecular Research, Vol.13, No.5, 446-452, October, 2005

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Surface Characteristics and Fibroblast Adhesion Behavior of RGD-Immobilized Biodegradable PLLA Films

Hyun Jung Jung , Kwang-Duk Ahn , Dong Keun Han^†, and Dong-June Ahn¹

Biomaterials Research Center, Korea Institute of Science and Technology, P. O. Box 131, Cheongryang, Seoul 130-650, Korea
¹Department of Chemical and Biological Engineering, Korea University, Seoul 136-701, Korea

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The interactions between the surface of scaffolds and specific cells play an important role in tissue engineering applications. Some cell adhesive ligand peptides including Arg-Gly-Asp (RGD) have been grafted into polymeric scaffolds to improve specific cell attachment. In order to make cell adhesive scaffolds for tissue regeneration, biodegradable nonporous poly(L-lactic acid) (PLLA) films were prepared by using a solvent casting technique with chloroform. The hydrophobic PLLA films were surface-modified by Argon plasma treatment and in situ direct acrylic acid (AA) grafting to get hydrophilic PLLA-g-PAA. The obtained carboxylic groups of PLLA-g-PAA were coupled with the amine groups of Gly-Arg-Asp-Gly (GRDG, control) and GRGD as a ligand peptide to get PLLAg-GRDG and PLLA-g-GRGD, respectively. The surface properties of the modified PLLA films were examined by various surface analyses. The surface structures of the PLLA films were confirmed by ATR-FTIR and ESCA, whereas the immobilized amounts of the ligand peptides were 138-145 pmol/cm2. The PLLA surfaces were more hydrophilic after AA and/or RGD grafting but their surface morphologies showed still relatively smoothness. Fibroblast adhesion to the PLLA surfaces was improved in the order of PLLA control < PLLA-g-PAA=PLLA-g-GRDG < PLLA-g-GRGD, indicating that PLLA-g-GRGD has the highest cell adhesive property.

Keywords:tissue engineering;PLLA film;plasma treatment;direct AA grafting;RGD immobilization;fibroblast adhesion.

[References]

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[2] Hubbell JA, Langer R, Chem. Eng. News, 13, 42 (1995)

[3] Alberts B, Bray D, Lewis J, Raff M, Roberts K, Warson JD, in Molecular Biology of the Cell, Garland Publishing, New York, p 971 (1994)

[4] Kim BS, Mooney DJ, TIBITECH, 16, 224 (1998)

[5] Vert M, Makromol. Chem. Makromol. Symp., 6, 109 (1986)

[6] Gilding DK, Reed AM, Polymer, 20, 1459 (1979)

[7] Hay DL, von Fraunhofer JA, Chegini N, Masterson BJ, J. Biomed. Mater. Res., 22, 179 (1988)

[8] Barrera DA, Zylstra E, Lansbury PT, Langer R, J. Am. Chem. Soc., 115, 11010 (1993)

[9] Barrera DA, Zylstra E, Lansbury PT, Langer R, Macromolecules, 28(2), 425 (1995)

[10] Gao J, Niklason L, Langer R, J. Biomed. Mater. Res., 42, 417 (1998)

[11] Chen G, Ushida T, Tateishi T, Macromol. Biosci., 2, 67 (2002)

[12] Nuutinen JP, Clerc C, Virta T, Tormala P, J. Biomater. Sci.-Polym. Ed., 13, 1325 (2002)

[13] Ma Z, Gao C, Gong Y, Shen J, Biomaterials, 24, 3725 (2003)

[14] Chim H, Ong JL, Schantz JT, Hutmacher DW, Agrawal CM, J. Biomed. Mater. Res., 65A, 327 (2003)

[15] Holy CE, Cheng C, Davies JE, Shoichet MS, Biomaterials, 22, 25 (2001)

[16] Riekerink MBO, Claase MB, Engbers GH, Grijpma DW, Feijen J, J. Biomed. Mater. Res., 65A, 417 (2003)

[17] Pierschbacher MD, Ruoslahti E, Nature, 309, 30 (1984)

[18] Hersel U, Dahman C, Kessle H, Biomaterials, 24, 4385 (2003)

[19] Shin HS, Jo SB, Mikos AG, Biomaterials, 24, 4353 (2003)

[20] Mooney DJ, Sano K, Kaufmann PM, Majahod K, Schloo B, Vacanti JP, Langer R, J. Biomed. Mater. Res., 37, 413 (1997)

[21] Massia SP, Hubbell JA, Anal. Biochem., 187, 292 (1990)

[22] Massia SP, Hubbell JA, J. Cell Biol., 114, 1089 (1991)

[23] Gumupderelioδlu M, Turkoδlu H, Biomaterials, 23, 3927 (2002)

[24] Cell Proliferation Reagent WST-1 technical bulletin, Roche Diagnostic GmbH, Germany.

[25] Bisson I, Kosinshi M, Ruault S, Gupta B, Hilborn J, Wurm F, Frey P, Biomaterials, 23, 3149 (2002)

[26] Holy CE, Cheong C, Davies JE, Shoichet MS, Biomaterials, 22, 25 (2001)

[27] Reikerink MBO, Claase MB, Engbers GH, Grijpma DW, Feijen J, J. Biomed. Mater. Res., 65A, 417 (2003)

[28] Kantlehner M, Schaffner P, Finsinger D, Meyer J, Jonczyk A, Diefenbach B, Nies B, Holzemann G, Goodman SL, Kessler H, Chem. Bio Chem., 1, 107 (2000)

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[31] Steffens GCM, Nothdurft L, Buse G, Thissen H, Hocher H, Klee D, Biomaterials, 23, 3523 (2002)

[32] Marletta G, Ciapetti G, Satriano C, Pagani S, Baldini N, Biomaterials, 26, 4793 (2005)

[33] Ma ZW, Gao CY, Juan J, Ji J, Gong YH, Shen JC, J. Appl. Polym. Sci., 85(10), 2163 (2002)

[34] Aksoy S, Tumturk H, Hasirci N, J. Biotechnol., 60, 37 (1998)

[35] Rezania A, Healy KE, J. Biomed. Mater. Res., 52, 595 (2000)

[36] Hubbell JA, Trends Polym. Sci., 2, 20 (1994)

[37] Hubbell JA, Bioresour. Technol., 13, 565 (1995)