In this study, of the efficient recovery and purification of lincomycin from the culture broth of Streptomyces lincolnensis L1245, we investigated how various factors, such as impurities, pH, and temperature, affected the recovery and purification in the resin process. XAD-4 resin absorbed the highest amount of lincomycin solution (14.9 ×104 μ/mL resin), but when a culture broth containing lincomycin was used, SIP1300 resin displayed the highest adsorption (13.8 × 104 μ/mL resin). When olive oil was present in the culture broth below 3.0 g/L, the degree of adsorption was above 12.6×104 μ/mL resin. However, when olive oil was at present above 9.0 g/L, the degree of adsorption decreased. The optimum pH for adsorbing lincomycin when using SIP1300 resin was 9.0. The adsorption amounts were similar at temperatures between 10 and 40 ℃. When VL/VR was 10 on the flow curve, the lincomycin present in the culture broth was completely adsorbed. On the other hand, when VL/VR was 60, SIP1300 resin adsorbed lincomycin at near saturation levels. We scaled the optimum conditions up to the use of a 100-L column. The amount adsorbed using SIP1300 resin under flow conditions was 12.5×104 μ/mL resin. The average yields of adsorption and elution, crude lincomycin-HC1, decoloration, and crystallization were 99.3, 95.7, 96.4, and 95.1 %, respectively.