Mortierella alpina was grown in a fed-batch culture using a 12-l jar fermenter with an initial 8-l working volume containing 20 g glucose l(-1) and 10 g corn-steep powder l(-1). Glucose was intermittently fed to give 32 g l(-1) at each time. The pH of culture was maintained using 14% (v/v) NH4OH, which also acted as a nitrogen source. A final cell density of 72.5 g l(-1) was reached after 12.5 days with a content of arachidonic acid (ARA) at 18.8 g l(-1). These values were 4 and 1.8 times higher than the respective values in batch culture. Our results suggest that the combined feeding of glucose and NH4+ to the growth of M. alpina could be applied for the industrial scale production of ARA.