The direct electron transfer of hemoglobin (Hb) was successfully realized on Au-zirconium phosphate nanocomposite (Au-ZrP) modified glassy carbon electrode (GCE) based on the synergistic effect between ZrP and An nanoparticles. The interaction between Hb and Au-ZrP was studied by UV-Vis spectroscopy, electrochemical impedance spectroscopy, and cyclic voltammetry. A couple of stable and well-defined redox peaks of Hb immobilized on Au-ZrP was observed at about -134 and 35 mV in pH 6.0 buffers. Moreover, the immobilized Hb showed excellent electrocatalysis to the reduction of H2O2, which was used to construct H2O2 sensors. The obtained sensor could determine H2O2 from 15 mu mol l(-1) to 0.48 mmol l(-1) with detection limit of 7.4 mu mol l(-1) (S/N= 3). Its apparent Michaelis-Menten constant, K-APP(M), was 0.65 mmol l(-1). (c) 2005 Elsevier B.V. All rights reserved.