Biotechnology Letters, Vol.27, No.20, 1591-1595, 2005
Controllable regioselective acylation of rutin catalyzed by enzymes in non-aqueous solvents
An efficient route to synthesize 3 ''- and 4'''-vinyl rutin esters has been developed by enzyme-catalyzed regioselective acylation of rutin with divinyl dicarboxylates in organic media. Alkaline protease from Bacillus subtilis provided 3 ''-O-substituted vinyl rutin esters in pyridine, and Novozym 435 gave 4'''-O-substituted vinyl rutin esters in tert-butanol.