맥주발효 폐액을 이용한 미생물 셀룰로오스 생산

박중곤; 현승훈; 안원술; Joong Kon Park; Seung Hoon Hyun; Won Sool Ahn

Korean Chemical Engineering Research, Vol.44, No.1, 52-57, February, 2006

Export Citation

맥주발효 폐액을 이용한 미생물 셀룰로오스 생산

Production of Bacterial Cellulose Using Waste of Beer Fermentation Broth

박중곤 ^†, 현승훈, 안원술 ¹

경북대학교 화학공학과, 701-701 대구시 북구 산격동 1370
¹계명대학교 화학공학과, 704-706 대구시 달서구 신당동 1000

Joong Kon Park^†, Seung Hoon Hyun, and Won Sool Ahn¹

Department of Chemical Engineering, Kyungpook National University, 1370, SanKyuk-dong, Buk-gu, Daegu 701-702, Korea
¹Department of Chemical Engineering, Kyemyung University, 1000, Shindang-dong, Dalseo-gu, Daegu 704-706, Korea

E-mail:

초록

에탄올과 함께 탄소, 질소원을 함유한 맥주 폐 발효액을 저렴한 대체배지로 사용하고 Gluconacetobacter hansenii PJK(KCTC 10505 BP) 균주를 이용하여 미생물셀룰로오스를 생산하였다. 맥주 폐 발효액은 기본배지보다도 탄소원과 질소원이 풍부하였으며 미량의 황과 4％ 이상의 에탄올을 함유하였다. 진탕배양에서 맥주 폐 발효액을 사용하여 생산된 셀룰로오스량은 기본배지를 사용하여 생산된 셀룰로오스량과 필적하였다. 교반배양에서는 셀룰로오스 생산균주(Cel+균주)가 셀룰로오스를 생산하지 못하는 균주(Cel. 돌연변이주)로 전환되는 률은 낮았지만 셀룰로오스 생산량은 감소하였다.

Bacterial cellulose (BC) was produced by Gluconacetobacter hansenii PJK (KCTC 10505 BP) strains using the waste of beer fermentation broth. It contained more C and N than a basal medium with a small amount of Sand more than 4％ ethanol. The amount of BC produced in a shaking culture using the waste of beer fermentation broth was nearly the same as that of a basal medium. The production of BC decreased in a shear stress field in a jar fermenter although the conversion of cellulose producing (Cel+) cells to non-cellulose producing (Cel.) mutants was not severe.This study showed that the waste of beer fermentation broth is an inexpensive carbon, nitrogen source with ethanol and thus a worthy substitute for the conventional medium for BC production.

Keywords:Bacterial Cellulose;Glucuronacetobacter Hansenii Pjk;Beer Fermentation Broth

[References]

Brown AJ, J. Chem. Soc., 49, 432 (1886)
Delmer DP, Annu. Rev. Plant Physiol. Plant Mol. Biol., 50, 245 (1999)
Ross P, Mayer R, Benziman M, Microbiol. Rev., 55, 35 (1991)
Matthysse AG, J. Bacteriol., 154, 906 (1983)
Rainer J, Luiz FF, Polym. Degrad. Stabil., 58, 101 (1998)
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Embuscado ME, Marks JS, BeMiler JN, Food Hydrocolloids, 8, 419 (1994)
Yoshino T, Asakura T, Toda K, J. Ferment. Bioeng., 81(1), 32 (1996)
Klemm D, Schumann D, Udhard U, Marsch S, Prog. Polym. Sci, 26, 1561 (2001)
Yamanaka S, Watanabe K, Kitamura N, Iguchi M, Mitsuhashi S, Nishi Y, Uryn M, J. Mater. Sci., 24, 3141 (1989)
Chanliud E, Gidley MJ, Plant J., 20, 25 (1999)
Bettocchi C, Delneri D, Signore S, Weng Z, Bruschi CV, Biochim. Biophys. Acta., 1336, 211 (1997)
Park SH, Yang YK, Hwang JW, Lee CS, Pyun YR, Kor. J. Appl. Microbiol. Biotechnol., 25, 598 (1997)
Ponyi T, Szabo L, Nagy T, Orosz L, Simpson PJ, Williamson MP, Gilbert HJ, J. Biochem., 39, 985 (2000)
Shibazaki H, Kuga S, Onabe F, Usuda M, J. Appl. Polym. Sci., 50, 965 (1993)
Vandamme EJ, De Baets S, Vanbaelen A, Joris K, DeWulf P, Polym. Degrad. Stabil., 59, 93 (1998)
Raghothama S, Simpson PJ, Szabo L, Nagy T, Gilbert HJ, Williamson MP, J. Biochem., 39, 978 (2000)
Sieger CHN, Kroon AGM, Batelaan JG, Van Ginkel CG, Carbohydr. Polym., 27, 137 (1995)
Yu XC, Atalla RH, J. Biological. Macromolecules, 19, 145 (1996)
Jeong YJ, Lee IS, Food Industry and Nutrition, 5, 25 (2000)
Yoshinaga F, Tonouchi N, Watanabe K, Biosci. Biotechnol. Biochem., 61, 219 (1997)
Park JK, Park YH, Jung JY, Biotechnol. Bioprocess. Eng., 8, 83 (2002)
Son HJ, Lee OM, Kim YG, Park YK, Lee SJ, Kor. J. Biotechnol. Bioeng., 15, 573 (2000)
Valla S, Kjosbakken J, J. Beneral Microb., 128, 1401 (1981)
Park JK, Jung JY, Park YH, Biotechnol. Lett., 25(24), 2055 (2003)

[Referenced By]

[Related Articles]

[1] Brown AJ, J. Chem. Soc., 49, 432 (1886)

[2] Delmer DP, Annu. Rev. Plant Physiol. Plant Mol. Biol., 50, 245 (1999)

[3] Ross P, Mayer R, Benziman M, Microbiol. Rev., 55, 35 (1991)

[4] Matthysse AG, J. Bacteriol., 154, 906 (1983)

[5] Rainer J, Luiz FF, Polym. Degrad. Stabil., 58, 101 (1998)

[6] Yamanaka S, Watanabe K, "Applications of Bacterial Cellulose in Cellulosic Polymers", pp. 207-215. In R Gillbert (ed), Cellulosic Polymers, Blends and Composites, Hanser Inc., Cincinnati, OH, USA.

[7] Embuscado ME, Marks JS, BeMiler JN, Food Hydrocolloids, 8, 419 (1994)

[8] Yoshino T, Asakura T, Toda K, J. Ferment. Bioeng., 81(1), 32 (1996)

[9] Klemm D, Schumann D, Udhard U, Marsch S, Prog. Polym. Sci, 26, 1561 (2001)

[10] Yamanaka S, Watanabe K, Kitamura N, Iguchi M, Mitsuhashi S, Nishi Y, Uryn M, J. Mater. Sci., 24, 3141 (1989)

[11] Chanliud E, Gidley MJ, Plant J., 20, 25 (1999)

[12] Bettocchi C, Delneri D, Signore S, Weng Z, Bruschi CV, Biochim. Biophys. Acta., 1336, 211 (1997)

[13] Park SH, Yang YK, Hwang JW, Lee CS, Pyun YR, Kor. J. Appl. Microbiol. Biotechnol., 25, 598 (1997)

[14] Ponyi T, Szabo L, Nagy T, Orosz L, Simpson PJ, Williamson MP, Gilbert HJ, J. Biochem., 39, 985 (2000)

[15] Shibazaki H, Kuga S, Onabe F, Usuda M, J. Appl. Polym. Sci., 50, 965 (1993)

[16] Vandamme EJ, De Baets S, Vanbaelen A, Joris K, DeWulf P, Polym. Degrad. Stabil., 59, 93 (1998)

[17] Raghothama S, Simpson PJ, Szabo L, Nagy T, Gilbert HJ, Williamson MP, J. Biochem., 39, 978 (2000)

[18] Sieger CHN, Kroon AGM, Batelaan JG, Van Ginkel CG, Carbohydr. Polym., 27, 137 (1995)

[19] Yu XC, Atalla RH, J. Biological. Macromolecules, 19, 145 (1996)

[20] Jeong YJ, Lee IS, Food Industry and Nutrition, 5, 25 (2000)

[21] Yoshinaga F, Tonouchi N, Watanabe K, Biosci. Biotechnol. Biochem., 61, 219 (1997)

[22] Park JK, Park YH, Jung JY, Biotechnol. Bioprocess. Eng., 8, 83 (2002)

[23] Son HJ, Lee OM, Kim YG, Park YK, Lee SJ, Kor. J. Biotechnol. Bioeng., 15, 573 (2000)

[24] Valla S, Kjosbakken J, J. Beneral Microb., 128, 1401 (1981)

[25] Park JK, Jung JY, Park YH, Biotechnol. Lett., 25(24), 2055 (2003)