Near-optimal control strategies for maximizing the production of induced foreign protein by recombinant bacteria, which were determined from optimal control simulation studies, were implemented using a two-liter bioreactor. A proportional-integral feedback controller with anticipatory action and a self-tuning controller with anticipatory action were simulated and compared for regulation of glucose concentration along an optimal singular are production strategy. The proportional-integral feedback control algorithm was implemented to suppress disturbances and model uncertainties while the anticipatory controller followed the computed singular are trajectories. Both simulation and experimental work proved that optimal control of the fed-batch system for induced foreign protein production can be carried out on-line.