Extraction and purification of eupatilin from Artemisia princeps PAMPANrecycling preparative HPLC

Youn-Woo Lee; Yinzhe Jin; Kyung Ho Row

Korean Journal of Chemical Engineering, Vol.23, No.2, 279-282, March, 2006

DOI10.1007/BF02705727 Export Citation

Extraction and purification of eupatilin from Artemisia princeps PAMPANrecycling preparative HPLC

Youn-Woo Lee , Yinzhe Jin¹, and Kyung Ho Row^{1, †}

School of Chemical and Biological Engineering and Institute of Chemical Processes, Seoul National University, San 56-1, Sillim-dong, Gwanak-gu, Seoul 151-744, Korea
¹Center for Advanced Bioseparation Technology, Department of Chemical Engineering, Inha University, 253 Yonghyun-dong, Nam-gu, Incheon 402-751, Korea

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A recycling preparative HPLC was used to extract and separate the eupatilin contained in Artemisia princepsPAMPAN, and the optimum operating condition was experimentally determined. Eupatilin was extracted by ethanolsolvent from the leaf and trunk of Artemisia princeps PAMPAN. The resulting solution was further partitioned withn-hexane, chloroform and ethyl acetate. The solution containing eupatilin was collected using a preparative as wellas analytical column, and it was identified by LC-CE-MS. In the experiment, the mobile phase consisted of water/aceto-nitrile/TFA=50/50/0.5 (vol%), and UV wavelength measured was 370nm. For analytical chromatography, the injectionvolume was 20μL and the flow rate was fixed at 1.0mL/min. The measured retention time for eupatilin was approxi-mately 8.6min in the above operating condition. For recycling preparative HPLC with commercially available GSA310A column at 1.5mL/min of the flow rate and 2mL of injection volume, the purity of the eupatilin was almost 100%after recycling twice.

Keywords:Eupatilin;Artemisia princeps PAMPAN;Recycling HPLC;Extraction

[References]

Ahn BO, Ryu BK, Ko JI, Oh TY, Kim SH, Kim WB, Yang J, Lee EB, Hahm KB, J. Appl. Pharmacol., 5, 165 (1997)
Choi YJ, Han SK, Chung ST, Row KH, Korean J. Chem. Eng., 21(4), 829 (2004)
Hahm KB, Kim J H, You BM, Kim YS, Cho SW, Yim H, Ahn BO, Kim WB, Pancreas, 17, 153 (1998)
Hong SP, Row KH, HWAHAK KONGHAK, 40(4), 488 (2002)
Kim SH, Kim SH, Lee SD, Kim WB, Lee MG, Kim ND, Res. Comm. Mole. Path. and Pharm., 97, 165 (1997)
Koshihara Y, Neichi T, Murota S, Lao A, Fujimoto Y, Tatsuno T, Febs Lett., 158, 41 (1983)
Lee SJ, Kor. J. Raw Med., 6, 75 (1975)
Lee SJ, Oh TY, Ahn BO, Cho H, Kim WB, Kim YB, Surh YJ, Hahm KB, Mutat. Res., in press
Lee YW, So MS, Lee JW, Chung ST, Row KH, Korean J. Chem. Eng., 13(6), 578 (1996)
Oh TY, Ryu BK, Ko JI, Ahn BO, Kim SH, Kim WB, Lee EB, Jin JH, Hahm KB, Pharm. Res., 20, 414 (1997)
Seo HJ, Park KK, Surh YJ, Programs and Abstracts of the 2nd International, 161, 2 (1999)
Seo HJ, Surh YJ, Mutation Res., 496, 191 (2001)

[Referenced By]

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[1] Ahn BO, Ryu BK, Ko JI, Oh TY, Kim SH, Kim WB, Yang J, Lee EB, Hahm KB, J. Appl. Pharmacol., 5, 165 (1997)

[2] Choi YJ, Han SK, Chung ST, Row KH, Korean J. Chem. Eng., 21(4), 829 (2004)

[3] Hahm KB, Kim J H, You BM, Kim YS, Cho SW, Yim H, Ahn BO, Kim WB, Pancreas, 17, 153 (1998)

[4] Hong SP, Row KH, HWAHAK KONGHAK, 40(4), 488 (2002)

[5] Kim SH, Kim SH, Lee SD, Kim WB, Lee MG, Kim ND, Res. Comm. Mole. Path. and Pharm., 97, 165 (1997)

[6] Koshihara Y, Neichi T, Murota S, Lao A, Fujimoto Y, Tatsuno T, Febs Lett., 158, 41 (1983)

[7] Lee SJ, Kor. J. Raw Med., 6, 75 (1975)

[8] Lee SJ, Oh TY, Ahn BO, Cho H, Kim WB, Kim YB, Surh YJ, Hahm KB, Mutat. Res., in press

[9] Lee YW, So MS, Lee JW, Chung ST, Row KH, Korean J. Chem. Eng., 13(6), 578 (1996)

[10] Oh TY, Ryu BK, Ko JI, Ahn BO, Kim SH, Kim WB, Lee EB, Jin JH, Hahm KB, Pharm. Res., 20, 414 (1997)

[11] Seo HJ, Park KK, Surh YJ, Programs and Abstracts of the 2nd International, 161, 2 (1999)

[12] Seo HJ, Surh YJ, Mutation Res., 496, 191 (2001)