Influence of water on membrane and anode performance was studied with steady-state and electrochemical impedance spectroscopy (EIS) measurements using a symmetrical cell with hydrogen on both sides. Both full-cell and half-cell measurements were performed. To obtain half-cell data a new reference electrode approach was demonstrated based on porous references in a four-electrode setup. A varying membrane resistance with current density was obtained using current interrupt and EIS measurements. The EIS measurements showed two semicircles at 10(4) Hz and 0.01-0.1 Hz, respectively. The first corresponds to hydrogen adsorption and the second to the water dependence of the electrode performance and membrane resistance. The low-frequency semicircle appears in a frequency range depending on the membrane thickness. The loop corresponding to the discharge of the double-layer capacitance through the Volmer reaction appears at frequencies too high to be experimentally measurable. The experimental data were in good agreement with the model developed in Part I of this paper. The model was also successfully fitted to experimental full cell data at different current densities and membrane thicknesses. The experiments confirmed that the low-frequency semicircle is attributed to the water dependence of both anode and membrane performance.