충전층 반응기내에서 고정된 Pseudomonas aeruginosa에 의한 Cd2+의 제거

최광수; 김철경

Clean Technology, Vol.8, No.4, 217-222, December, 2002

Removal of Cadmium Ion (Cd2+) by Pseudomonas aeruginosa Immobilized in Ca-Alginate Gel Beads in Packed-Bed Column Reactor

최광수, 김철경

초록

본 연구에서는 수직 원통형 충전반응기(packed-bed column reactor)내에 균체가 고정화된 Ca-alginate bead를 넣어 카드뮴이온을 제거하는데 있어 반응기내로 유입되는 유속, 초기농도 등에서 카드뮴 이온(Cd2+) 제거하는데 최적 조건을 찾고자 하였다. 사용된 균주(Pseudomonas aeruginosa ATCC 27853)는 한국과학기술원 유전공학센터 유전자은행(KCTC)으로부터 구입하였고, Bead로 사용된 Ca-alginate bead는 Sodium Alginate에 균체와 CaCl2를 섞어 제조한 것을 사용하였다. 실험 결과 동일 조건일 때 유속이 30 mL/ hr > 45 mL/ hr > 60 mL/ hr의 순서로 제거율이 좋았고 유입속도의 변화에도 불구하고 각 유속에서 50 ppm > 100 ppm > 200 ppm > 300 ppm의 순으로 좋은 제거율을 보였다.

The effects of initial cadmium ion concentrations (50, 100, 200, 300ppm), and feeding velocities (30, 45, 60mL/hr) on the removal ratio of cadmium ion by Pseudomonas aeruginosa ATCC 27853 immobilized in Ca-alginae gel beads in a packed-bed column reactor were investigated at operating temperature 37°C. The removal ratio of cadmium ion with variable initial concrntration was decreased in the following oredr : 50 ppm > 100 ppm > 200 ppm > 300 ppm. The optimum removal conditions of cadmium ion by Pseudomonas aeruginosa ATCC 27853 were initial concentration 50ppm, feeding velocity 30mL/hr.

[References]

Charkrabarty AM, Plasmid Assisted Molecular Breeding, 21, 4 (1982)
Stanlake GF, Finn RK, Isolation and Characterization of Pentachlorophenol Degrading Bacterium, 4 (1982)
Kelda GK, Maier WJ, Kinetics of Microbial Growth of Pentachlorophenol, 49 (1985)
Williams D, Lay JD, Bacteriological Review, 41, 1 (1977)
Halling PJ, Dunnill P, Enzyme Microb. Technol., 2, 2 (1980)
Munnecke DM, Biotechnol. Bioeng., 23, 1813 (1981)
Linko YY, Rohjola L, FERS Letters., 62, 77 (1976)
McGhee JE, Rovert WD, Appl. Environ. Microbiol., 44, 19 (1982)
White CA, Kennedy WF, Enzyme Microb. Technol., 2, 82 (1980)
Bollmeier JP, Biotechnol. Bioeng., 21, 2303 (1979)
Peter SJ, Blunt KW, Physical studies on cell immobilization using Calcium Alginate Gels, 21, 2155 (1979)
최석순, Pseudomonas aeruginosa를 이용한 카드뮴 이온(Cd2+)의 제거, 석사학위논문, 성균관대학교 (1990)
Cheetham PHJ, Blut KV, Bucke C, Biotechnol. Bioeng., 21, 2155 (1979)

[1] Charkrabarty AM, Plasmid Assisted Molecular Breeding, 21, 4 (1982)

[2] Stanlake GF, Finn RK, Isolation and Characterization of Pentachlorophenol Degrading Bacterium, 4 (1982)

[3] Kelda GK, Maier WJ, Kinetics of Microbial Growth of Pentachlorophenol, 49 (1985)

[4] Williams D, Lay JD, Bacteriological Review, 41, 1 (1977)

[5] Halling PJ, Dunnill P, Enzyme Microb. Technol., 2, 2 (1980)

[6] Munnecke DM, Biotechnol. Bioeng., 23, 1813 (1981)

[7] Linko YY, Rohjola L, FERS Letters., 62, 77 (1976)

[8] McGhee JE, Rovert WD, Appl. Environ. Microbiol., 44, 19 (1982)

[9] White CA, Kennedy WF, Enzyme Microb. Technol., 2, 82 (1980)

[10] Bollmeier JP, Biotechnol. Bioeng., 21, 2303 (1979)

[11] Peter SJ, Blunt KW, Physical studies on cell immobilization using Calcium Alginate Gels, 21, 2155 (1979)

[12] 최석순, Pseudomonas aeruginosa를 이용한 카드뮴 이온(Cd2+)의 제거, 석사학위논문, 성균관대학교 (1990)

[13] Cheetham PHJ, Blut KV, Bucke C, Biotechnol. Bioeng., 21, 2155 (1979)