Kinetic modeling for chromatographic separation of cytosine monophosphate and uracil monophosphate

Yong Chen; Weixing Dai; Xuesong Liu; Yiyu Cheng; Haibin Qu

Korean Journal of Chemical Engineering, Vol.23, No.5, 784-788, September, 2006

DOI10.1007/BF02705928 Export Citation

Kinetic modeling for chromatographic separation of cytosine monophosphate and uracil monophosphate

Yong Chen, Weixing Dai, Xuesong Liu, Yiyu Cheng, and Haibin Qu^†

Pharmaceutical Informatics Institute, Department of Chinese Medicine Science & Engineering, Zhejiang University, Hangzhou 310027, China

E-mail:

Kinetic modeling for preparative chromatography is a topic of present interest in the fine chemicals and pharmaceutical industries. In this study, chromatographic separation of the two nucleotides CMP and UMP was simulated by the equilibrium-dispersive (ED) model, and the adsorption isotherms in the ED model were determined by the inverse method. Prediction performance of the model was validated under three different kinds of conditions and the importance of selecting isotherms was discussed in detail. Excellent agreement was achieved with the experimental band profiles and the prediction of the ED model. The ED model with bi-Langmuir isotherm was especially suitable for simulating chromatographic separation of CMP and UMP. The error of prediction by the ED model with bi-Langmuir isotherm was about 9.4 times smaller than that with Langmuir isotherm.

Keywords:Kinetic Modeling;Chromatographic Separation;Equilibrium-Dispersive Model;Adsorption Isotherms;CMP and UMP

[References]

Choi YJ, Han SK, Chung ST, Row KH, Korean J. Chem. Eng., 21(4), 829 (2004)
Choi YS, Lee JW, Koo YM, Row KH, Choi DK, Korean J. Chem. Eng., 17(6), 625 (2000)
Czok M, Guiochon G, Anal. Chem., 62, 189 (1990)
Felinger A, Cavazzini A, Guiochon G, J. Chromatogr. A, 986, 207 (2003)
Felinger A, Zhou D, Guiochon G, J. Chromatogr. A, 1005, 35 (2003)
Guiochon G, J. Chromatogr. A, 965, 129 (2002)
Guiochon G, Shirazi SG, Katti AM, Fundamentals of preparative and nonlinear chromatography, Academic Press, San Diego (1994)
Ji QM, Zhang SS, Wu YJ, Analytical Laboratory, 18, 83 (1999)
Kaczmarski K, Antos D, J. Chromatogr. A, 756, 73 (1996)
Ma Z, Guiochon G, Comput. Chem. Eng., 15, 415 (1991)
Morgan E, Burton KW, Chemometrics Intell. Lab. Syst., 8, 97 (1990)
Rosen JB, J. Chem. Phys., 20, 387 (1952)
Seidel-Morgenstern A, J. Chromatogr. A, 1037, 255 (2004)
Zhu J, Katti AM, Guichon G, J. Chromatogr., 552, 71 (1991)
Zupan J, Gasteiger J, Neural networks for chemists: introduction, VCH, New York (1993)

[1] Choi YJ, Han SK, Chung ST, Row KH, Korean J. Chem. Eng., 21(4), 829 (2004)

[2] Choi YS, Lee JW, Koo YM, Row KH, Choi DK, Korean J. Chem. Eng., 17(6), 625 (2000)

[3] Czok M, Guiochon G, Anal. Chem., 62, 189 (1990)

[4] Felinger A, Cavazzini A, Guiochon G, J. Chromatogr. A, 986, 207 (2003)

[5] Felinger A, Zhou D, Guiochon G, J. Chromatogr. A, 1005, 35 (2003)

[6] Guiochon G, J. Chromatogr. A, 965, 129 (2002)

[7] Guiochon G, Shirazi SG, Katti AM, Fundamentals of preparative and nonlinear chromatography, Academic Press, San Diego (1994)

[8] Ji QM, Zhang SS, Wu YJ, Analytical Laboratory, 18, 83 (1999)

[9] Kaczmarski K, Antos D, J. Chromatogr. A, 756, 73 (1996)

[10] Ma Z, Guiochon G, Comput. Chem. Eng., 15, 415 (1991)

[11] Morgan E, Burton KW, Chemometrics Intell. Lab. Syst., 8, 97 (1990)

[12] Rosen JB, J. Chem. Phys., 20, 387 (1952)

[13] Seidel-Morgenstern A, J. Chromatogr. A, 1037, 255 (2004)

[14] Zhu J, Katti AM, Guichon G, J. Chromatogr., 552, 71 (1991)

[15] Zupan J, Gasteiger J, Neural networks for chemists: introduction, VCH, New York (1993)