Journal of Industrial and Engineering Chemistry, Vol.12, No.5, 777-782, September, 2006
Lipase Immobilization on Silica Gel Using a Cross-linking Method
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The objective of this study was to develop a suitable method for immobilizing lipase. Although many investigators have studied lipase immobilization, detailed optimization of each step for lipase immobilization using cross-linking methods has been lacking. Therefore, we focused on establishing and optimizing each step of the immobilization procedure to improve procedural efficiencies. Lipase produced from Rhizopus oryzae was immobilized on silica gel using a cross-linking method. The immobilization procedures, such as the pretreatment of silica gel, silanization, crosslinking, glutaraldehyde modification, and elimination of unreated aldehyde groups, were all optimized. Furthermore, the stability of the immobilized lipase and its suitability for reuse were also evaluated.
- Murty VR, Bhat J, Muniswaran PKA, Biotechnol. Biopro. Eng., 7, 57 (2002)
- Cha SH, Kim JC, Lim JS, Yoon CS, Koh JH, Chang HI, Kim SW, J. Ind. Eng. Chem., 12(1), 115 (2006)
- Balcao VM, Paiva AL, Malcata FX, Enzyme Microb. Technol., 18(6), 392 (1996)
- Tischer W, Wedekind F, Top. Curr. Chem., 200, 95 (1999)
- Kim DJ, Shin DH, Hur BK, Kim EK, J. Microbiol. Biotechnol., 10, 836 (2000)
- Byun JW, Lee YS, J. Ind. Eng. Chem., 10(2), 283 (2004)
- Soares CMF, Santana MHA, Zanin GM, de Castro HF, Biotechnol. Prog., 19(3), 803 (2003)
- Cowan DA, Daniel RM, Biotechnol. Bioeng., 24, 2053 (1982)
- Cestari AR, Vieira EFS, Simoni JD, Airoldi C, Thermochim. Acta, 348(1-2), 25 (2000)
- Park SW, Kim YI, Chung KH, Kim SW, Proc. Biochem., 37, 153 (2001)
- Kwon DY, Rhee JS, JAOCS, 63, 89 (1986)
- Park SW, Kim YI, Chung KH, Kim SW, J. Microbiol. Biotechnol., 11, 199 (2001)
- Bradford MM, Anal. Biochem., 72, 248 (1976)
- Aravind S, Stephen JK, Patrick IO, Jacobson KB, Jonathan W, Michel JD, Enzyme Microb. Technol., 19, 74 (1999)
- Nanci A, Wuest JD, Peru L, Brunet P, Sharma V, Zalzal S, McKee MD, J. Biomed. Mater. Res., 40, 324 (1998)
- Makino K, Maruo SI, Morita Y, Takeuchi Y, Biotechnol. Bioeng., 31, 617 (2004)
- Park SW, Lee JW, Hong SI, Kim SW, Appl. Biochem. Biotechnol., 104(3), 185 (2003)
- Chen H, Hsieh YL, Biotechnol. Bioeng., 90, 405 (2004)
- Ye P, Xu ZK, Wang ZG, Wu J, Deng HT, Seta P, J. Mol. Catal., 32, 115 (2005)