A cyclodextrin glucanotransferase (CGTase) gene from Bacillus sp. TS1-1 was isolated and cloned into Escherichia coli. Starting from TTG codon, there was an open reading frame composed of 2163 bp (721 amino acids). The NE, terminal position encoded a 46-amino acid of a signal peptide and followed by the mature enzyme (675 amino acids). The deduced amino acid sequence of the mature CGTase from Bacillus sp. TS1-1 exhibited 98.7% homology with 96% identity to the CGTase sequence from alkalophilic Bacillus sp. 1-1. The recombinant CGTase of Bacillus sp. TS1-1 expressed in E. coli was successfully purified to homogeneity using ammonium sulfate precipitation, followed by alpha-cyclodextrin-boundepoxy-activated Sepharose 6B affinity chromatography. The purified CGTase enzymes exhibited a single band with molecular weight of 75 kDa on SDS-PAGE. Biochemical characterization of the enzyme shows an optimum temperature of 60 degrees C and optimum pH of 6.0. The enzyme was stable between pH 7 and 9 and temperature up to 70 degrees C. The K-m and V-max values calculated were 0.52 mg/ml and 54.35 mg of beta-cyclodextrin/ml/min. The yield of the products from soluble starch as the substrate were 86% for beta-cyclodextrin and 14% for gamma-cyclodextrin after 24 h incubation at 60 degrees C, without adding any selective agent. The total beta-CD produced under the conditions mentioned above was 3.65 g/l. (c) 2005 Published by Elsevier Inc.