Chiral selective cation-exchange membrane (CSCM) and chiral selective anion-exchange membrane (CSAM) were prepared by blending poly(vinyl alcohol) (PVA) as basic material, P-cyclodextrin (P-CD) as chiral selector, and ion-exchange materials. Chiral selective capacities of these membranes were evaluated by electrodialysis resolution experiment. Electrodialysis separation of DL-4-hydroxyphenylglycine (HPG) racemic mixture showed that the chiral selective membranes had resolution effect. In acid medium, D-HPG(+) took the precedence over L-HPG(+) in passing through the CSCM and the selectivity alpha(D/L) of two kinds of CSCM were 1.21 and 1.34, respectively. In base medium, L-HPG- took the lprecedence over D-HPG- in passing through the CSAM and the selectivity alpha(D/L) was 0.84. (c) 2005 Elsevier B.V. All rights reserved.