Novel self-assembled V2O5 bundles with highly ordered superstructures and spindle-like morphology were synthesized by a rapid high-yielding sonochemical method. The as-prepared samples were characterized by X-ray diffraction, a field-emission scanning electron microscope, and a transmission electron microscope. The spindle-like V2O5 bundles are composed of several tens of homogeneous nanowires with diameters of 30-50 nm and lengths of 3-7 mu m. A sensitive resonance light scattering method for the detection of bovine serum albumin (BSA) based on the self-assembled V2O5 bundles was developed. The results of the polarized resonance light scattering demonstrated that the Cabannes factor for the V2O5 bundles-BSA aggregates was BSA concentration-dependent.