A platinized carbon paste prepared via electrodeposition had a preferential electrocatalytic action toward H2O2. Therefore, we have developed a new amperometric glucose biosensor based on the immobilization of glucose oxidase on to the electrochemically platinized carbon paste. The proposed biosensor is free of potential interferences due to its cathodic detection of glucose at the potential of 0.0 V (vs. Ag/AgCl). It also shows acceptable analytical performance in terms of linearity (6 x 10(-5) to 1.2 x 10(-2) M, r = 0.998), detection limit (2 x 10(-5) M), response time (20-30 s), reproducibility (RSD = 4.4%), and storage life (t (0.80) = 45 days). All these advantages of the biosensor raise potential possibilities for its medical or other biotechnical applications.