Biotechnology Letters, Vol.28, No.19, 1595-1600, 2006
Enhanced production of recombinant nattokinase in Bacillus subtilis by the elimination of limiting factors
By systematic investigation, glutamate and a mixture of metal ions were identified as factors limiting the production of nattokinase in Bacillus subtilis. Consequently, in medium supplemented with these materials, the recombinant strain secreted 4 times more nattokinase (260 mg l(-1)) than when grown in the unsupplemented medium.