This paper deals with injection characteristics using different fuels at different fuel temperatures. The fuels under consideration are neat biodiesel from rapeseed oil and some blends with diesel as well as neat mineral diesel D2. The fuel and fuel temperature influences are investigated experimentally in the mechanically controlled diesel fuel injection M system. At first, attention is focused on the injection characteristics, especially on fuelling, mean injection rate, mean injection pressure, injection timing, injection delay, and injection duration, which influence the most important engine characteristics. Furthermore, the influence of fuel temperature is investigated. On the basis of the measurements of pressure drop through the fuel filter, the minimum fuel temperature for safe engine operation is determined.