Micellization of three didodecyl dicationic dibromide gemini surfactants with different methylene spacer lengths, 12-s-12,2Br(-) where s = 3- 5 methylene groups, has been investigated in water-ethylene glycol, EG, mixtures with weight percentages of EG up to 50%. Subsequently, effects of the addition of the organic solvent on the micellar growth of these surfactants and on the surfactant concentration range where sphere-to-rod transitions occur were studied by means of steady-state and time-resolved fluorescence quenching and spectroscopic measurements. Results show that an increase in the weight percentage of ethylene glycol added to aqueous 12-s-12,2Br(-) (s = 3-5) micellar solutions causes the sphere-to-rod transition to occur at higher surfactant concentrations than in pure water. The diminution in the average aggregation number, N-agg, when wt % EG increases, provoked by the decrease in the interfacial Gibbs energy contribution to Delta G degrees(M), is the main factor responsible for this observation. The decrease in N-agg is accompanied by a decrease in the ionic interactions and the extra packing contribution to the deformation of the surfactants tails, making formation of cylindrical micelles less favorable. Besides, an increase in the solvent content and polarity of the interfacial region does not favor formation of direct ion pairs, decreasing the tendency of micelles to grow.