Biotechnology Letters, Vol.29, No.1, 105-110, 2007
Pseudomonas stutzeri as a novel biocatalyst for pyruvate production from DL-lactate
Pseudomonas stutzeri SDM oxidized DL-lactic acid (25.5 g l(-1)) into pyruvic acid (22.6 g l(-1)) over 24 h. Both NAD(+)- independent D-lactate dehydrogenase and NAD+- independent L-lactate dehydrogenase were found for the first time in the bioconversion of lactate to pyruvate based on the enzyme activity assay and proteomic analysis.