A magnetically stabilized fluidized bed (MSFBs) utilizing a transverse magnetic field was used to retain cells from cell suspension. The magnetic field permits bed expansion without mixing of the magnetic particles. The bed porosity increased by 75% when the magnetic field intensity increases to 110 mT. The effect of the magnetic field, suspension flow rate, bed height, initial concentration, and pH on the breakthrough curves was studied. According to the experimental results, increasing the initial concentration, flow rate, and pH leads to early breakthrough and inefficient deposition. Additionally, increasing the field intensity and bed height delays the breakthrough point.