Aspartic acid, also known as aspartate, the name of its anion, is one of the 20 natural proteinogenic amino acids which are the building blocks of proteins. Aspartic acid is a major excitatory amino acid in the mervous system. It is of paramount importance in the metabolism during construction of other amino acid and biochemicals in the citric acid cycle. A rapid and sensitive chemiluminescence (CL) method was developed for the determination of aspartic acid. The method is based on the CL reaction of aspartic acid with Ce (IV) and Tris (2,2'-bipyridyl) ruthenium (II), Ru (bipy)₃²⁺. After optimization of the different experimental parameters, a calibration graph was obtained over a concentration range of 0.13 μg/ml - 13.3 μg/ml with minimum detectability of 0.075 μg/ml (S/N = 3). The correlation coefficient was 0.991 (n = 7) with a relative standard deviation (% R.S.D) of 1.51%.