Encapsulation of CdSe/ZnS Quantum Dots in Poly(ethylene glycol)-Poly(D,L-lactide) Micelle for Biomedical Imaging and Detection

Yong-Kyu Lee; Suk Min Hong; Jin Su Kim; Jeong Hyuk Im; Hyun Su Min; Elango Subramanyam; Kang Moo Huh; Sung-Woo Park

Macromolecular Research, Vol.15, No.4, 330-336, June, 2007

Yong-Kyu Lee, Suk Min Hong, Jin Su Kim, Jeong Hyuk Im¹, Hyun Su Min¹, Elango Subramanyam¹, Kang Moo Huh^{1, †}, and Sung-Woo Park²

Department of Chemical and Biological Engineering, Chungju National University, Chungbuk 380-702, Korea
¹School of Applied Chemistry and Biological Engineering, Chungnam National University, Daejeon 305-764, Korea
²Department of Scientific Criminal Investigation, Chungnam National University, Daejeon 305-764, Korea

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Luminescent CdSe/ZnS QDs, with emission in the red region of the spectrum, were synthesized and encapsulated in poly(ethylene glycol)-poly(D,L-lactide) diblock copolymer micelles, to prepare water-soluble, biocompatible QD micelles. PEG-PLA diblock copolymers were synthesized by ring opening polymerization of D,L-lactide, in the presence of methoxy PEG as a macroinitiator. QDs were encapsulated with PEG-PLA polymers using a solid dispersion method in chloroform. The resultant polymer micelles, with encapsulated QDs, were characterized using various analytical techniques, such as UV-Vis measurement, light scattering, fluorescence spectroscopy, transmission electron microscopy (TEM) and atomic forced microscopy (AFM). The polymer micelles, with encapsulated QDs, were spherical and showed diameters in the range of 20-150 nm. The encapsulated QDs were highly luminescent, and have high potential for applications in biomedical imaging and detection.

Keywords:CdSe/ZnS QDs;PEG-PLA diblock copolymer;polymer micelle;encapsulation

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[3] Chan WCW, Maxwell DJ, Gao X, Bailey RE, Han M, Nie S, Curr. Opin. Biotechnol., 13, 40 (2002)

[4] Wu X, Liu H, Liu J, Haley KN, Treadway JA, Larson JP, Ge N, Peale F, Bruchez MP, Nat. Biotechnol., 21, 41 (2003)

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[7] Alivisatos AP, Science, 271(5251), 933 (1996)

[8] Murray CB, Norris DJ, Bawendi MG, J. Am. Chem. Soc., 115, 8706 (1993)

[9] Hines MA, Gnyotsionnest P, J. Phys. Chem., 100(2), 468 (1996)

[10] Bruchez M, Moronne M, Gin P, Weiss S, Alivisatos AP, Science, 281(5385), 2013 (1998)

[11] Wang Y, Tang ZY, Correa-Duarte MA, Pastoriza-Santos I, Giersig M, Kotov NA, Liz-Marzan LM, J. Phys. Chem. B, 108(40), 15461 (2004)

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[15] Murray CB, Norris DJ, Bawendi MG, J. Am. Chem. Soc., 115, 8706 (1993)

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[17] Yu WW, Wang YA, Peng X, Chem. Mater., 15, 4300 (2003)

[18] Yu WW, Peng X, Angew. Chem.-Int. Edit., 41, 2368 (2002)

[19] Battaglia D, Peng X, Nano Lett., 2, 1027 (2002)

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[21] Bailey RE, Nie SM, J. Am. Chem. Soc., 125(23), 7100 (2003)

[22] Kim S, Lim YT, Soltesz EG, De Grand AM, Lee J, Nakayama A, Parker JA, Mihaljevic T, Laurence RG, Dor DM, Cohn LH, Bawendi MG, Frangioni JV, Nat. Biotechnol., 22, 93 (2004)

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