The electrophoresis of two identical spheres moving along the axis of a long cylindrical pore under the conditions of low surface potential and weak applied electric field is investigated. The geometry considered allows us to examine simultaneously the effects of boundary and the presence of a nearby entity on the behavior of a particle. The influences of the separation distance between two spheres, the thickness of a double layer, the ratio (radius of sphere/radius of pore), and the charged conditions on the surfaces of the spheres and the pore on the mobility of a particle are investigated. Several interesting results that are not reported in the literature are observed. For instance, although for the case of two positively charged spheres in an uncharged pore the qualitative behavior of a sphere depends largely on its size relative to that of a pore and the thickness of the double layer, this might not be the case when two uncharged spheres are in a positively charged pore. In addition, in the latter, the mobility of a sphere increases with the increases in the separation distance between two spheres, and this effect is pronounced when the ratio (radius of sphere/radius of pore) takes a medium value or the thickness of the double layer is either sufficiently thin or sufficiently thick.